Generator

Part:BBa_K1179023:Design

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)


CAG_Acyl-TyA-rtTA3-6xHis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2379
    Illegal PstI site found at 2628
    Illegal PstI site found at 2650
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
    Illegal PstI site found at 2379
    Illegal PstI site found at 2628
    Illegal PstI site found at 2650
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal BglII site found at 2479
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2379
    Illegal PstI site found at 2628
    Illegal PstI site found at 2650
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2379
    Illegal PstI site found at 2628
    Illegal PstI site found at 2650
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2312
    Illegal BsaI.rc site found at 2664
    Illegal BsaI.rc site found at 3117


Design Notes

Not applicable

Source

The TyA protein is a yeast viral protein and when added with the Acylation tag it acts as an exosome export protein, allowing the eGFP to be exported when fused to the TyA. Constructed by performing a gateway reaction with CAG and Acyl-TyA-rtTA-His.

References